Restriction Endonuclease Mapping of pUHl inBacillus subtilis(natto)
نویسندگان
چکیده
منابع مشابه
Endonuclease-mediated long PCR and its application to restriction mapping.
The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. Applications for PCR have been extended significantly by the development of "long" PCR, a technique that makes it possible to amplify DNA fragments up to 40 kb in length. This article describes two novel applications of the long PCR technique, one which simplifies restriction mapping and another which en...
متن کاملChimeric restriction endonuclease.
Fok I restriction endonuclease recognizes the nonpalindromic pentadeoxyribonucleotide 5'-GGATG-3'.5'-CATCC-3' in duplex DNA and cleaves 9 and 13 nt away from the recognition site. Recently, we reported the presence of two distinct and separable domains within this enzyme: one for the sequence-specific recognition of DNA (the DNA-binding domain) and the other for the endonuclease activity (the c...
متن کاملRestriction glycosylases: involvement of endonuclease activities in the restriction process
All restriction enzymes examined are phosphodiesterases generating 3'-OH and 5'-P ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. Whether its restriction activity involves endonucleolytic cleavage remains unclear. One report on this enzyme, R.PabI from a hyperthermophile, ascribed the breakage to high temperature while another...
متن کاملRestriction endonuclease mapping of unintegrated proviral DNA of Kirsten murine sarcoma virus.
Linear unintegrated Kirsten murine sarcoma virus DNA synthesized after acute infection of NIH/3T3 cells or by detergent-disrupted virions, was studied by restriction enzyme cleavage and agarose gel electrophoresis. Labelled DNA (cDNA) synthesized by reverse transcription of a virion RNA template was used to detect viral DNA sequences by filter hybridization. The sites of cleavage for eleven enz...
متن کاملIsolation and identification of restriction endonuclease BsiSI.
BseBI, an isoschizomer of BstNI (1) has been purified from Bacillus stearothermopilus species. BseBI recognises the sequence 5'...CCWGG...3' (W=A or T) and cleaves between C and W. The enzyme was purified using the following Chromatographic steps: 1. Blue -Sepharose F3GA, 2. Heparin -Sepharose, 3. DEAE-Sepharose. The enzyme was free of contaminating nuclease activity. After 5-fold overdigestion...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1983
ISSN: 0002-1369
DOI: 10.1080/00021369.1983.10865790